Etude des mécanismes inflammatoires dans l’accident vasculaire cérébral


Recently we developed and validated a method of cellular imaging of inflammation using high resolution MRI. This approach was based on macrophage tracking using intravenous injection of ultrasmall superparamagnetic nanoparticles of iron oxides (USPIO). From the preliminary results, it was possible to define the dynamics of post-ischemic inflammation in mice [1]. This approach is now being completed by an MRI study of human cerebral infarction. The objective is to study the relationship between the decrease in the MRI signal induced by USPIOs and the clinical and pathological parameters [2] .

Figure. Correlation of gradient-echo (GRE) signals with immunohistology. A- GRE MRI 72h post-pMCAO and i.v. injection of USPIO. Note the hyposignal around the lesion, the contralateral corpus callosum and the ipsilateral peri-ventricular area. B- Double staining with Prussian blue and F4/80 of the right (ipsilateral) ventricle. Iron-stained microglia/macrophages are clearly visible along the lateral wall, in correlation with the hyposignal observed in GRE images (A). C- Prussian blue staining for iron in the contralateral corpus callosum of the corresponding slice. Positive staining was observed around cell nuclei, which suggests cytoplasm uptake (insert). D- F4/80 immunostaining for mouse microglia/macrophages in the contralateral corpus callosum. Note the F4/80+ brown cells, in spatial agreement with iron+ cells (B) and MRI hyposignal (A). E- F4/80 immunostaining of a non-operated control mouse in the left corpus callosum, with no positive staining. F- Double staining with Prussian blue and F4/80: magnification of B. Microglia/macrophages as identified by their brown color and typical ramified shape, were also blue-stained, suggesting USPIO intra-cellularity.